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Reconstruct 3D objects using GIMP 3.0 and ImageJ.
A Practical Guide to 3D Reconstruction from Serial Sections: Using GIMP3, ImageJ, and Mathematica
by Yasushi Miki (Author), Daniel K. Gladish (Author)
ISBN-13: 979-8268447194
Publication date: October 25, 2025
Kindle, Paperback

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Re-Evaluation of Vascular Histogenesis in the Root Tips of Selected Species in the Poaceae Using New Methods: Analysis of the Plerome, Vascular Initials, Pericycle and Late-Maturing Metaxylem Vessels
Yasushi Miki, Susumu Saito, Teruo Niki and Daniel K. Gladish
Plants 2024, 13(6), 910; https://doi.org/10.3390/plants13060910

Figure 9. Characteristics of the primary root tip procambia of four taxa (1–4) in the Poaceae as seen in enhanced section images and 3D constructions. (A) Views of the basipetal face of the vascular initials layers, (B) oblique perspective of 3D constructions of the central initials and plerome cells (shades of green), (C) transverse images of the VC at 100 µm from the RCJ, and (D) 3D images from the primary root tips of the LMX(s) and two pericycle cell files associated with their plerome and vascular initials layer. Scale bar = 50 µm.
謹賀新年2024

3Dオブジエクト手法による根先端域での篩部篩管の起源と発達の解析
仁木輝緒 ・ 幹 康 ・ 斉藤 進
(同) ミキ音響イメージプロセッシング部門
第58回根研究集会(2023年11月3–5日,姫路)

連続薄切片を用いた3Dオブジェクト作成プロトコル【追補】
連続薄切片を用いた3Dオブジェクト作成プロトコル

Amazon公式サイトで「連続切片」で検索してください. オンデマンド(ペーパーバック)2023年7月25日発売 Kindle版2023年7月19日発売
Improved image processing for 3D virtual object construction from serial sections reveals tissue patterns in root tips of Zea mays
Yasushi Miki, Susumu Saito, Teruo Niki and Daniel K. Gladish
Applications in Plant Sciences, vol. 11, July 2023
https://doi.org/10.1002/aps3.11531

